lipod293 transfection reagent (SignaGen)
90
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SignaGen
lipod293 transfection reagent
Lipod293 Transfection Reagent, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipod293%E2%84%A2+transfection+reagent/lipod293/pmc11287164__pnas__2303642121__sapp-9-0-5
Average 90 stars, based on 1 article reviews
Lipod293 Transfection Reagent, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipod293%E2%84%A2+transfection+reagent/lipod293/pmc11287164__pnas__2303642121__sapp-9-0-5
Average 90 stars, based on 1 article reviews
lipod293 transfection reagent - by Bioz Stars,
2026-09
90/100 stars
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shRNA:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Plasmid Preparation:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Transfection:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Control:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Purification:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Recombinant:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Red Blood Cell Lysis:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Activation Assay:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Membrane:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Staining:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Isolation:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using DC Protein Assay:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Enzyme-linked Immunosorbent Assay:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Expressing:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Amplification:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Software:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Imaging:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Spectrophotometry:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Lysis:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Protease Inhibitor:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Western Blot:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using SYBR Green Assay:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Produced:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Positive Control:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Cell Culture:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Modification:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using Construct:Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: Cells were either transfected with 1 μg of LRRK2 constructs, co-transfected with 1 μg of LRRK2 and 100 ng RL1d-GFP construct, or co-transfected with 1 μg of pCMV and 100 ng of GFP-RILPL1, RILPL1-GFP or RILPL1-miniSOG constructs, along with 4 μl of Article Title: LRRK2 causes centrosomal deficits via phosphorylated Rab10 and RILPL1 at centriolar subdistal appendages Article Snippet: A549 cells were cultured in glass-bottom MatTek dishes (MatTek Life Sciences, P35G-0-14-C) and transfected with RILPL1-miniSOG-HA using Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using Article Title: Robust eIF4B levels undermine invasive growth and immune evasion mechanisms in murine triple negative breast cancer models Article Snippet: Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using |
